atcc scrc 1041tm  (ATCC)

Journal: Nature Communications

Article Title: HCMV infection disrupts barrier functions and promotes epithelial–mesenchymal transition in a cholangiocyte organoid model

doi: 10.1038/s41467-026-68962-8

Figure Lengend Snippet: a hiPSC-derived cholangiocyte-like cell (CLC) organoids were infected with HCMV TB40/E-eGFP at an MOI of 1. Representative images of mock- and HCMV-infected CLC organoids on day 10 PI are shown at 40× and 100× magnifications. b Number and c size of mock- and HCMV-infected CLC organoids on days 4 and 10 PI. For b , n = 132 and 102 for Day 4 PI Mock and HCMV; 208, 135 for Day 10 PI Mock and HCMV, respectively, measured from three biological replicates. For c , n = 6 biological replicates. Flow cytometry results showing percentage of GFP + cells in mock- and HCMV-infected CLC organoids on day 10 PI, shown as d dot plots or as a e column graph ( n = 6 biological replicates). f qPCR analysis of number of intracellular HCMV DNA copies per cell in mock-infected, HCMV-infected GFP - and GFP + cells, and Hs27 fibroblasts at 48 h PI. n = 9, 9, 6, and 4 biological replicates, respectively. g Representative images of in situ hybridisation of HCMV DNA probe (yellow), with GFP signals from HCMV-infected CLC organoid on day 10 PI or mock control, counterstained with DAPI (blue). Column graph shows quantification of percentage of probe⁺ and GFP⁺ cells ( n = 5 biological replicates). h Immunofluorescence staining of HCMV late protein pp28 (red) with GFP signals (green); i column graph shows percentage of pp28⁺, GFP⁺, and double-positive cells. j Supernatant (sup) from mock- and HCMV-infected CLC organoids were co-cultured with HFF-1 cells for 2 days and monitored for GFP signals ( n = 4 biological replicates). k RT-qPCR analysis of HCMV gene expression ( UL44, UL55, UL83, UL122, LAcmvIL10 , and LUNA ) in mock-infected, HCMV-GFP¯, and HCMV-GFP + cells from infected CLC organoids ( n = 6 biological replicates for UL44, UL55, UL83 , and UL122 ; n = 3 biological replicates for LAcmvIL10 and LUNA ). Scale bars with dimensions are shown. Data in graphs show the mean ± SD. Boxplots show centre line as median with box boundaries as 25 th to 75 th percentile, and whiskers as minima to maxima. One-way ANOVA was performed for ( b , c , f ). Student’s paired two-tailed t -test for ( e ). P values for b , P = 0.03 between Day 4 PI Mock vs HCMV, P < 0.001 between Day 10 PI Mock vs HCMV; for c , 0.62 between Day 4 PI Mock vs HCMV, P < 0.001 between Day 10 PI Mock vs HCMV; for e , P < 0.001 between Mock and HCMV; for ( f , P < 0.001 between GFP - and GFP + ; for k , for UL55 , UL83 , UL122 , UL138 , LAcmvIL10 , LUNA , between HCMV-GFP − and HCMV-GFP + , P = 0.01, 0.02, 0.01, <0.001 < 0.001, <0 . 001, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001. Also see Figs. and .

Article Snippet: HFF-1 (#SCRC-1041) and Hs27 (#CRL-1634) cells are both human foreskin fibroblasts and were purchased from ATCC.

Techniques: Derivative Assay, Infection, Flow Cytometry, In Situ, Hybridization, Control, Immunofluorescence, Staining, Cell Culture, Quantitative RT-PCR, Gene Expression, Two Tailed Test

Journal: Macromolecular Bioscience

Article Title: Lignin Nanoparticles Containing Cobalt‐Cyanine Complexes: Potential Multifunctional Platforms for Photoacoustic Imaging and Photothermal Treatment of Bacterial Biofilms in Chronic Wounds

doi: 10.1002/mabi.202500532

Figure Lengend Snippet: Cell compatibility of CoPc‐Lig NPs. A) Cell viability of fibroblasts (black bars) and keratinocytes (white bars) exposed to different concentrations of CoPc‐Lig NPs for 24 h. B) Confocal images showing cell internalization of CoPc‐Lig NPs (0.5 mg/mL) by fibroblasts and keratinocytes. The dark areas in brightfield images represent CoPc‐Lig NPs, while the fluorescence channels show cell nuclei (Blue), and cell cytoskeleton (green).

Article Snippet: Bacterial strains ( Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 9027) and human fibroblast cell line (ATCC‐SCRC‐1041, HFF‐1) were purchased from the American Type Culture Collection (ATCC LGC Standards, Italy).

Techniques: Fluorescence